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On the quantitative determination of liver ferritin in the rat

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
65
Issue
3
Identifiers
DOI: 10.1016/0009-8981(75)90258-2
Disciplines
  • Biology

Abstract

Abstract The measurement of liver ferritin is usually performed after homogenization of liver, heating the homogenate and centrifugation. Because ferritin is stable to 80° this protein and its iron are recovered in the supernatant. We found that this procedure resulted in losses of ferritin so we developed a method to measure ferritin protein in the unheated rat liver homogenate. Total liver ferritin iron could be calculated with use of the ferritin protein and ferritin iron values as measured in the supernatant after heating the liver homogenate.

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