Affordable Access

A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A.

Authors
  • A R Quesada
  • J D McGivan
Publication Date
Nov 01, 1988
Source
PMC
Keywords
License
Unknown

Abstract

A rapid method for the functional reconstruction of amino acid transport from liver plasma-membrane vesicles using the neutral detergent decanoyl-N-glucamide ('MEGA-10') is described. The method is a modification of that previously employed in this laboratory for reconstitution of amino acid transport systems from kidney brush-border membranes [Lynch & McGivan (1987) Biochem. J. 244, 503-508]. The transport activities termed 'System A', 'System N', and 'System L' are all reconstituted. The reconstitution procedure is rapid and efficient and is suitable as an assay for transport activity in studies involving membrane fractionation. By using this reconstitution procedure, System A transport activity was partially purified by lectin-affinity chromatography.

Report this publication

Statistics

Seen <100 times