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A Novel Screening Method for Isolating Exopolysaccharide-Deficient Mutants

Authors
  • Minghsun Liu
  • Juan E. González
  • Laura B. Willis
  • Graham C. Walker
Publisher
American Society for Microbiology
Publication Date
Nov 01, 1998
Source
PMC
Keywords
License
Unknown

Abstract

A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria.

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