A microsatellite linkage map of Drosophila mojavensis

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A microsatellite linkage map of Drosophila mojavensis

BioMed Central
Publication Date
May 26, 2004
  • Biology
  • Ecology
  • Medicine


1471-2156-5-12.fm ral ss BioMed CentBMC Genetics Open AcceResearch article A microsatellite linkage map of Drosophila mojavensis Regina Staten1,2, Sheri Dixon Schully1 and Mohamed AF Noor*1 Address: 1Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803 USA and 2Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808 USA Email: Regina Staten - [email protected]; Sheri Dixon Schully - [email protected]; Mohamed AF Noor* - [email protected] * Corresponding author Abstract Background: Drosophila mojavensis has been a model system for genetic studies of ecological adaptation and speciation. However, despite its use for over half a century, no linkage map has been produced for this species or its close relatives. Results: We have developed and mapped 90 microsatellites in D. mojavensis, and we present a detailed recombinational linkage map of 34 of these microsatellites. A slight excess of repetitive sequence was observed on the X-chromosome relative to the autosomes, and the linkage groups have a greater recombinational length than the homologous D. melanogaster chromosome arms. We also confirmed the conservation of Muller's elements in 23 sequences between D. melanogaster and D. mojavensis. Conclusions: The microsatellite primer sequences and localizations are presented here and made available to the public. This map will facilitate future quantitative trait locus mapping studies of phenotypes involved in adaptation or reproductive isolation using this species. Background Evolutionary biologists have struggled to determine the number and types of genetic changes that lead to specia- tion. Recent advances in molecular techniques facilitate a more thorough investigation into these issues. For exam- ple, by mapping quantitative trait loci (QTLs) affecting interesting traits, we can explore the genetic basis of phe- notypic variation between two populations that may lead to reproductive isolation. One hallmark specie

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