A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain

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A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain

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BioMed Central
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PMC
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  • Biology
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Abstract

1471-213X-5-14.fm ral ss BioMed CentBMC Developmental Biology Open AcceResearch article A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain Adrienne E McKee†1,2, Emmanuel Minet†2,3, Charlene Stern2, Shervin Riahi2, Charles D Stiles2,4 and Pamela A Silver*1,2 Address: 1Department of Systems Biology, Harvard Medical School, Boston, MA 02115 USA, 2Department of Cancer Biology, The Dana-Farber Cancer Institute, Boston, MA 02115 USA, 3URBC-FUNDP, 61 rue de Bruxelles, 5000 Namur, Belgium and 4Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115 USA Email: Adrienne E McKee - [email protected]; Emmanuel Minet - [email protected]; Charlene Stern - [email protected]; Shervin Riahi - [email protected]; Charles D Stiles - [email protected]; Pamela A Silver* - [email protected] * Corresponding author †Equal contributors Abstract Background: In eukaryotic cells, RNA-binding proteins (RBPs) contribute to gene expression by regulating the form, abundance, and stability of both coding and non-coding RNA. In the vertebrate brain, RBPs account for many distinctive features of RNA processing such as activity-dependent transcript localization and localized protein synthesis. Several RBPs with activities that are important for the proper function of adult brain have been identified, but how many RBPs exist and where these genes are expressed in the developing brain is uncharacterized. Results: Here we describe a comprehensive catalogue of the unique RBPs encoded in the mouse genome and provide an online database of RBP expression in developing brain. We identified 380 putative RBPs in the mouse genome. Using in situ hybridization, we visualized the expression of 323 of these RBP genes in the brains of developing mice at embryonic day 13.5, when critical fate choice decisions are made and at P0, when major s

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