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A general method for the purification of restriction enzymes.

Authors
  • P J Greene
  • H L Heyneker
  • F Bolivar
  • R L Rodriguez
  • M C Betlach
  • A A Covarrubias
  • K Backman
  • D J Russel
  • R Tait
  • H W Boyer
Publication Date
Jul 01, 1978
Source
PMC
Disciplines
  • Biology
License
Unknown

Abstract

An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.

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