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A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.

Authors
  • W Jäger
  • J Kalinowski
  • A Pühler
Publication Date
Apr 01, 1997
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.

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