Affordable Access

deepdyve-link
Publisher Website

A Comparative Study of Iron Uptake Mechanisms in Marine Microalgae: Iron Binding at the Cell Surface Is a Critical Step

Authors
  • Robert Sutak1
  • Hugo Botebol1
  • Pierre-Louis Blaiseau1
  • Thibaut Léger1
  • François-Yves Bouget1
  • Jean-Michel Camadro1
  • Emmanuel Lesuisse1
Type
Published Article
Journal
PLANT PHYSIOLOGY
Publisher
American Society of Plant Biologists
Volume
160
Issue
4
Pages
2271–2284
Identifiers
DOI: 10.1104/pp.112.204156
Source
CdV-UPMC
Keywords
License
White

Abstract

We investigated iron uptake mechanisms in five marine microalgae from different ecologically important phyla: the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana, the prasinophyceae Ostreococcus tauri and Micromonas pusilla, and the coccolithophore Emiliania huxleyi. Among these species, only the two diatoms were clearly able to reduce iron, via an inducible (P. tricornutum) or constitutive (T. pseudonana) ferrireductase system displaying characteristics similar to the yeast (Saccharomyces cerevisiae) flavohemoproteins proteins. Iron uptake mechanisms probably involve very different components according to the species, but the species we studied shared common features. Regardless of the presence and/or induction of a ferrireductase system, all the species were able to take up both ferric and ferrous iron, and iron reduction was not a prerequisite for uptake. Iron uptake decreased with increasing the affinity constants of iron-ligand complexes and with increasing ligand-iron ratios. Therefore, at least one step of the iron uptake mechanism involves a thermodynamically controlled process. Another step escapes to simple thermodynamic rules and involves specific and strong binding of ferric as well as ferrous iron at the cell surface before uptake of iron. Binding was paradoxically increased in iron-rich conditions, whereas uptake per se was induced in all species only after prolonged iron deprivation. We sought cell proteins loaded with iron following iron uptake. One such protein in O. tauri may be ferritin, and in P. tricornutum, Isip1 may be involved. We conclude that the species we studied have uptake systems for both ferric and ferrous iron, both involving specific iron binding at the cell surface. There are two main strategies for iron uptake by ter-restrial microorganisms and plants, and both have been characterized in the yeast Saccharomyces cerevisiae (for re-view, see Kosman, 2003; Philpott and Protchenko, 2008; Blaiseau et al., 2010). The first is the reductive mechanism of uptake. Extracellular ferric complexes are dissociated by reduction via transplasma membrane electron transfer catalyzed by specialized flavohemoproteins (Fre). In

Report this publication

Statistics

Seen 106 times