Affordable Access

Mutants ofBacillus stearothermophiluslacking NAD-linkedl-lactate dehydrogenase

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Keywords
  • Fermentation
  • Ethanol Production
  • Fluoropyruvate Resistance
  • Thermophile
Disciplines
  • Biology

Abstract

Abstract Bacillus stearothermophilus produces low levels of ethanol following fermentation of glucose at temperatures in excess of 60°C. A major by-product is l-lactic acid. To improve ethanol yields mutants of this organism were isolated which lacked NAD-linked l-lactate dehydrogenase, the enzyme responsible for lactic acid production. This was achieved by selection for resistance to a suicide substrate, fluoropyruvate. Such mutants no longer produced lactic acid and yields of ethanol from glucose were increased by at least a factor of two.

There are no comments yet on this publication. Be the first to share your thoughts.