Abstract A national survey was conducted during July to September 2009 to determine TB prevention activities, problems and support needed of Thai hospitals. More than 90% of hospitals established TB isolation policy, provided guidelines for caring TB patients, TB prevention and environmental management, established screening system for TB in the OPD. A half of hospitals had problems with isolation rooms and difficulties in screening TB cases in the OPD. Support needed included consultation on structure and ventilation systems, personnel training, national TB prevention and TB screening guideline. Strengthening TB prevention activities, providing expert consultation and national guidelines may help hospitals improve their TB prevention activities.