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Combined supercritical fluid chromatographic tests to improve the classification of numerous stationary phases used in reversed-phase liquid chromatography

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1189
Identifiers
DOI: 10.1016/j.chroma.2007.12.062
Keywords
  • Stationary Phases
  • Ods
  • Polar-Embedded
  • Hydrophilic Endcapping

Abstract

Abstract In this paper, we present a combination of a key-solute test based on retention and separation factors of large probe solutes (carotenoid pigments) and a quantitative structure-retention relationship analysis based on the retention factors of small probe solutes (aromatic compounds), both performed in supercritical fluid chromatography, to investigate the different chromatographic behaviour of octadecylsiloxane-bonded stationary phases of all sorts: classical, protected against silanophilic interactions or not, containing polar groups (endcapping groups or embedded groups). The results indicate that the two approaches chosen (carotenoid test and solvation parameter model) are complementary and provide precise information on the chromatographic behaviour of ODS phases. The applicability of the classification to the selection of stationary phases is evidenced with some examples of separations.

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