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Changes in the protein profile ofQuercus ilexleaves in response to drought stress and recovery

Authors
Journal
Journal of Plant Physiology
0176-1617
Publisher
Elsevier
Publication Date
Volume
166
Issue
3
Identifiers
DOI: 10.1016/j.jplph.2008.05.008
Keywords
  • Drought Stress
  • Holm Oak Proteome
  • Mass Spectrometry
  • Quercus Ilex
Disciplines
  • Agricultural Science
  • Biology
  • Computer Science
  • Physics

Abstract

Summary To characterize the molecular response of holm oak to drought stress and its capacity to recover 9-month-old Quercus ilex seedlings were subjected to three treatments for a 14-d period: (i) continuous watering to field capacity (control plants, W), (ii) no irrigation (drought treatment, D), and (iii) no irrigation for 7 d followed by a watering period of 7 d (recovery treatment, R). In drought plants, leaf water potential decreased from −0.72 (day 0) to −0.99 MPa (day 7), and −1.50 MPa (day 14). Shoot relative water content decreased from 49.3% (day 0) to 47.7% (day 7) and 40.8% (day 14). Photosystem II quantum yield decreased from 0.80 (day 0) to 0.72 (day 7) and 0.73 (day 14). Plants subjected to water withholding for 7 d reached, after a 7-d rewatering period, values similar to those of continuously irrigated control plants. Changes in the leaf protein pattern in response to drought and recovery treatments were analyzed by using a proteomic approach. Twenty-three different spots were observed when comparing the two-dimensional electrophoresis profile of control to both drought and recovered plants. From these, 14 proteins were identified from tryptic peptides tandem mass spectra by using the new Paragon algorithm present in the ProteinPilot software. The proteins identified belong to the photosynthesis, carbohydrate and nitrogen metabolism, and stress-related protein functional categories.

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