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Methylated blocked 5′ terminal sequences of sea urchin embryo messenger RNA classes containing and lacking poly(A)

Authors
Journal
Cell
0092-8674
Publisher
Elsevier
Publication Date
Volume
9
Issue
4
Identifiers
DOI: 10.1016/0092-8674(76)90041-6

Abstract

Abstract Sea urchin embryo mRNAs of three distinct classes—histone mRNA, nonhistone mRNA containing poly(A), “[+A] mRNA,” and nonhistone mRNA lacking poly(A), “[−A] mRNA”—all contain blocked 5′ terminal sequences in which 7-methylguanosine is linked 5′-5′ via a triphosphate bridge to a 2′-0-methylated nucleotide. Only one general type of 5′ terminal structure, 7mGpppX mpYp, is present. Additional 2′-0-methylation in the Y residue has not been found either in early or late stage embryos. A substantial proportion of the polyribosomal mRNAs in all three classes contain this blocked structure. Whereas both classes of nonhistone mRNAs have internal base methylations, histone mRNAs lack such modifications.

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