Affordable Access

Publisher Website

Transcriptome Sequencing to Detect Gene Fusions in Cancer

Authors
Journal
Nature
0028-0836
Publisher
Nature Publishing Group
Publication Date
Volume
458
Issue
7234
Identifiers
DOI: 10.1038/nature07638
Keywords
  • Article

Abstract

Recurrent gene fusions, typically associated with hematological malignancies and rare bone and soft tissue tumors1, have been recently described in common solid tumors2–9. Here we employ an integrative analysis of high-throughput long and short read transcriptome sequencing of cancer cells to discover novel gene fusions. As a proof of concept we successfully utilized integrative transcriptome sequencing to “re-discover” the BCR-ABL1 10 gene fusion in a chronic myelogenous leukemia cell line and the TMPRSS2-ERG 2,3 gene fusion in a prostate cancer cell line and tissues. Additionally, we nominated, and experimentally validated, novel gene fusions resulting in chimeric transcripts in cancer cell lines and tumors. Taken together, this study establishes a robust pipeline for the discovery of novel gene chimeras using high throughput sequencing, opening up an important class of cancer-related mutations for comprehensive characterization.

There are no comments yet on this publication. Be the first to share your thoughts.