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A method for the stabilization of toluidine blue vital staining for histological and ultrastructural studies

Authors
Journal
Micron (1969)
0047-7206
Publisher
Elsevier
Publication Date
Volume
14
Issue
4
Identifiers
DOI: 10.1016/0047-7206(83)90007-9
Disciplines
  • Chemistry

Abstract

Abstract A method previously developed to stabilize methylene blue vital staining has been applied to toluidine blue, a basic dye with a closely related chemical structure commonly used as a vital stain for the screening of oral and esophageal lesions. The stain was made stable by adding 1% sodium silicotungstate to an aldehyde fixative (formaldehyde or glutaraldehyde). Sections were prepared for light and electron microscopy.

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