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Some Observations on the Bleaching Effect of Ethylenediaminetetraacetic Acid on Green Barley Leaves 12

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Green barley leaves (Hordeum vulgaris) floated on the surface of 0.05 m ethylenediamine disodium tetraacetate, EDTA-2Na, pH 7.0 and exposed to light (5000 lux) at 25° exhibited a marked bleaching (EDTA-bleaching) visible to the naked eye and paralleled by a striking reduction in content of chlorophylls a and b. This loss of color did not occur in controls which were treated with H2O instead of EDTA (water controls). In darkness the leaves in the water controls were bleached while EDTA-treated leaves retained all their color.

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