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2-Aminothiophenols as fluorogenic reagents for aromatic aldehydes

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
282
Issue
3
Identifiers
DOI: 10.1016/0003-2670(93)80127-7

Abstract

Abstract Substituted 2-aminothiophenols (7 species) were evaluated for fluorescence derivatization reagents of aromatic aldehydes in spectrofluorimetry and liquid chromatography. Of the compounds, 2-amino-5-methoxythiophenol was the most favourable in practical use. The reagent reacts selectively with aromatic aldehydes in acidic media within 70 min at 50°C. The fluorescent products from benzaldehyde and 4-hydroxybenzaldehyde are shown to be 2-phenyl-6-methoxybenzothiazole and 2-(4-hydroxyphenyl)-6-methoxybenzothiazole, respectively. The detection limits for aromatic aldehydes in the spectrofluorometric method were 7–80 pmol ml −1 depending on the aldehydes. The fluorescent derivatives of aromatic aldehydes can be separated by reversed-phase chromatography and their detection limits ( S/N = 3) are 0.1–0.4 pmol on column.

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