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Cloning and expression of the levanase gene inAlcaligenes eutrophusH16 enables the strain to hydrolyze sucrose

Authors
Journal
Journal of Biotechnology
0168-1656
Publisher
Elsevier
Publication Date
Volume
10
Identifiers
DOI: 10.1016/0168-1656(89)90072-2
Keywords
  • Alcaligenes Eutrophus
  • Recombinant Dna
  • Sucrose Hydrolysis
  • Levanase
Disciplines
  • Biology
  • Engineering

Abstract

Abstract Genetic engineering methods were used to enhance the substrate spectrum of Alcaligenes eutrophus H16, a poly-β-hydroxybutyric acid (PHB) producer. Using parts of the vector pMMB33 and a 2.5 kb DNA fragment of the Bacillus subtilis chromosome a plasmid was constructed bearing the gene for levanase, an enzyme able to hydrolyze various saccharides. After transfer of the levanase gene by triparental conjugation, the gene, controlled by its own Bacillus subtilis promoter, is expressed in Alcaligenes eutrophus H16 and enables the strain to hydrolyze sucrose. However, growth on sucrose is limited; i.e. the sucrose is not transported efficiently into the cell and/or the levanase is not secreted into the medium.

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