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HPLC separation of corticosterone metabolites and its use in following corticosterone metabolism and transport in the isolated rat hepatocyte

Authors
Journal
Journal of Steroid Biochemistry
0022-4731
Publisher
Elsevier
Publication Date
Volume
29
Issue
1
Identifiers
DOI: 10.1016/0022-4731(88)90388-3

Abstract

Abstract Incubating isolated rat hepatocytes with tritium labelled and unlabelled corticosterone at 37°C resulted in the rapid appearance of at least nine corticosterone metabolites. A quick, relatively easy, and quantitative high performance liquid Chromatographic (HPLC) method was used to separate these metabolites and follow their rate of appearance both intra- and extracellulariy. We found different intraand extracellular amounts of each metabolite at a particular time and this suggested that some metabolites were more available for transport than others.

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