Affordable Access

Publisher Website

HPLC separation of corticosterone metabolites and its use in following corticosterone metabolism and transport in the isolated rat hepatocyte

Authors
Journal
Journal of Steroid Biochemistry
0022-4731
Publisher
Elsevier
Publication Date
Volume
29
Issue
1
Identifiers
DOI: 10.1016/0022-4731(88)90388-3

Abstract

Abstract Incubating isolated rat hepatocytes with tritium labelled and unlabelled corticosterone at 37°C resulted in the rapid appearance of at least nine corticosterone metabolites. A quick, relatively easy, and quantitative high performance liquid Chromatographic (HPLC) method was used to separate these metabolites and follow their rate of appearance both intra- and extracellulariy. We found different intraand extracellular amounts of each metabolite at a particular time and this suggested that some metabolites were more available for transport than others.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments