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Sonication as an aid for handling geraldton wax (Chamelaucium uncinatum) pollen

International Society for Horticultural Science
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Pollen in Chamelaucium species is exuded from the anthers in an oily substance and deposited onto the pollen presenter. Pollen in oil was sonicated in 20% sucrose at 20 watts, then spun down at 8,000 rpm for 60 s. Pollen germination was reduced by sonication (from 50% to 30%), and pollen tube length was reduced by about one third in 2 of the 3 lines tested. When sonicated pollen was used on the stigma, good germination was observed; by 48 h there were as many pollen tubes at the base of the style as when using unsonicated pollen. Overall 11.5% seed set was observed using sonicated pollen in intraspecific crosses of C. uncinatum, and 7% in C. uncinatum x megalopetalum crosses.

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