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In vitroprimary culture of embryonic cells from the boll weevil,Anthonomus grandis

Authors
Journal
Journal of Invertebrate Pathology
0022-2011
Publisher
Elsevier
Publication Date
Volume
17
Issue
1
Identifiers
DOI: 10.1016/0022-2011(71)90129-7

Abstract

Abstract When embryonic cells of boll weevils, Anthonomus grandis, were grown in primary cultures, large aggregates of cells developed the first week, pulsing actions were strong, and multiplication occurred. Then for 4–7 weeks, the tissues grew unattached, formed large aggregates, and continued to pulse and enlarge. Characteristically, the tissues developed spherical, membranous outgrowths, and cells developed peripherally and became multilayered; possibly, they possess an interior membrane. These latter growths resembled midgut cells.

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