Abstract The aim of the study was to evaluate the clonality of 21 strains of Enterococcus faecalis associated with arthritis and amyloid arthropathy by multilocus sequence typing (MLST). The strains originated from five countries. Fifteen of the 21 strains demonstrated the same sequence type, ST82, including a reference amyloid arthropathy strain from the Netherlands. Some of the demonstrated sequence types (ST36, ST59 and ST82) have also been described from human clinical samples while other types are reported for the first time. The results confirm previous observations that outbreaks of amyloid arthropathy seem to be clonally related and indicate a wide distribution of the predominant sequence type which was demonstrated in four countries in Europe and in the USA.