Abstract 1. 1. The effects of concentrate feeding on the levels and pattern of distribution of carbohydrases in bovine intestine and pancreas were investigated. 2. 2. No remarkable difference was noticed in the pattern of distribution of the carbohydrases along the bovine intestine, which was mostly confined to the proximal part of the small intestine. 3. 3. The concentrate feeding, however, highly affected the levels of carbohydrases in the mucosa, luminal contents and the pancreas. Their levels slightly decreased in the mucosal tissue and significantly increased in the luminal contents. In the pancreas, the level of amylase decreased and that of disaccharidases increased. 4. 4. Based on the presence of higher levels of activities of carbohydrases in the luminal contents, supported by the concentrate-induced increase in their levels, it is argued that the site of carbohydrate digestion, including disaccharides, in the small intestine, is the luminal contents.