Affordable Access

Publisher Website

Evidence That Acetyl-CoA Carboxylase Isoforms Play Different Biological Roles in H9c2 Cardiaomyocyte

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
248
Issue
3
Identifiers
DOI: 10.1006/bbrc.1998.8991
Disciplines
  • Biology

Abstract

Abstract The present work was performed to identify the possible roles of acetyl-CoA carboxylase isoforms (ACC-α and ACC-β). Two forms show 70% amino acid identity, but N-terminal regions share no homology, indicating that these may be uniquely related to the specific role of each ACC form. Thus, we investigated whether introduction of the exogenous ACC N-terminus into H9c2 cardiomyocytes that express both ACC forms causes a noticeable change in a specific pathway of fatty acid metabolism. The effect of ACC-α N-terminus overexpression was specific to the fatty acid synthesis rate resulting in an 80% induction, whereas overexpression of the ACC-β N-terminus increased fatty acid oxidation rate 50% without affecting the fatty acid synthesis rate. These results suggest that ACC-α and β are involved in the regulation of fatty acid synthesis and oxidation, respectively, and that the N-terminus plays an important role in the process. We further demonstrated that novel proteins specifically bound to the ACC N-terminus. This interaction may mediate the involvement of each ACC form in different cellular activities.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Evidence that acetyl-CoA carboxylase isoforms play...

on Biochemical and Biophysical Re... Jul 30, 1998

Rapid increase of cytosolic content of acetyl-CoA...

on Experimental & molecular medic... Jun 30, 1998

The crystal structure of DL-acetylleucine N-methyl...

on Acta crystallographica. Sectio... Sep 15, 1969

Synthesis and crystal structures of (C8h8)Nd(C5H9C...

on Journal of Organometallic Chem... Jan 01, 1995
More articles like this..