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Conjugation in Escherichia coli: Recombination Events in Terminal Regions of Transferred Deoxyribonucleic Acid

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  • Microbial Physiology And Metabolism


An investigation of recombination events occurring in zygotes formed during conjugation has been carried out. The frequencies with which donor markers situated close to the origin are recovered indicates an obligatory interaction between the recipient chromosome and a region on the donor chromosome adjacent to the leading end. The partial exclusion from recombinants of some of the most proximal markers studied, however, indicates that this interaction does not occur at the free extremity of the transferred deoxyribonucleic acid. Alternate models to explain these facts are presented.

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