Affordable Access

Publisher Website

Stimulation by oleic acid of incorporation of L-[4,5-3H]leucine into very low density lipoprotein apoprotein by the isolated perfused rat liver

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
132
Issue
1
Identifiers
DOI: 10.1016/0006-291x(85)90983-0
Disciplines
  • Biology

Abstract

Abstract Livers from normal fed or fasted (24h) rats were perfused in vitro to determine whether fatty acid affects the biosynthesis of very low density lipoprotein (VLDL) apoprotein. Oleate stimulated VLDL triacylglycerol output and increased incorporation of L-[4,5- 3H]leucine into VLDL apoprotein in both the fed and fasted groups. The increased incorporation of [ 3H]leucine was mainly into VLDL-apoprotein E. The total mass of VLDL apoprotein secreted was also stimulated by oleate proportionately. These data suggest that fatty acids may stimulate hepatic synthesis and/or secretion of the VLDL apoproteins and that apo E, may be required for the formation and secretion of triacylglycerol in the VLDL.

There are no comments yet on this publication. Be the first to share your thoughts.