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Detection strategies for bioassays based on enzyme-amplied lanthanide luminescence

University of Twente
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  • Biology


Strategies to expand the range of applicability of the EALL detection scheme are presented within this thesis. First, it is described how the EALL method can be employed for the on-line monitoring of enzymatic conversions. The development of a new EALL system for the determination of one hydrolytic and one redox enzyme is presented, and the catalytic properties of lanthanide(III) ions towards the ester cleavage of certain model compounds are investigated.

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