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Sendai virus-based RSV vaccine protects against RSV challenge in an in vivo maternal antibody model

Authors
Journal
Vaccine
0264-410X
Publisher
Elsevier
Publication Date
Volume
32
Issue
26
Identifiers
DOI: 10.1016/j.vaccine.2014.03.088
Keywords
  • Respiratory Syncytial Virus Vaccine
  • Sendai Virus
  • Maternal Antibody Model
  • Neutralizing Antibodies
  • Protective Immunity

Abstract

Abstract Respiratory syncytial virus (RSV) is the cause of significant morbidity and mortality among infants, and despite decades of research there remains no licensed vaccine. SeVRSV is a Sendai virus (SeV)-based live intranasal vaccine that expresses the full length RSV fusion (F) gene. SeV is the murine counterpart of human parainfluenza virus type 1. Given that the target population of SeVRSV is young infants, we questioned whether maternal antibodies typical of this age group would inhibit SeVRSV vaccine efficacy. After measuring SeV- and RSV-specific serum neutralizing antibody titers in human infants, we matched these defined titers in cotton rats by the passive transfer of polyclonal or monoclonal antibody products. Animals were then vaccinated with SeVRSV followed by a 3 month rest period to allow passively transferred antibodies to wane. Animals were finally challenged with RSV to measure the de novo vaccine-induced immune responses. Despite the presence of passively-transferred serum neutralizing antibodies at the time of vaccination, SeVRSV induced immune responses that were protective against RSV challenge. The data encourage advancement of SeVRSV as a candidate vaccine for the protection of children from morbidity and mortality caused by RSV.

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