Affordable Access

Publisher Website

Rapid fluorescence-based measurement of neutrophil migration in vitro

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
213
Issue
1
Identifiers
DOI: 10.1016/s0022-1759(98)00016-7
Keywords
  • Leukocyte
  • Neutrophil
  • Migration
  • Chemotaxis
  • Interleukin-8
  • C5A
  • Fluorescence
  • Calcein Am

Abstract

Abstract We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the fluorescence-based assay was more rapid, less labor intensive, and more sensitive. This new assay is a significant advance in the measurement of leukocyte migration in vitro.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Rapid fluorescence-based measurement of neutrophil...

on Journal of Immunological Metho... Apr 01, 1998

Fluorescence-based assays for in vitro analysis of...

on Methods in Molecular Biology 2009

Improving fluorescence-based assays for the in vit...

on Molecular biotechnology March 2002

Automation and validation of a rapid method to ass...

on Cytometry. Part B, Clinical cy... September 2008
More articles like this..