Affordable Access

Non-coding RNAs in Saccharomyces cerevisiae: what is the function?

Authors
Publication Date
Disciplines
  • Biology

Abstract

RNA UK 2012 907 Non-coding RNAs in Saccharomyces cerevisiae: what is the function? Jian Wu, Daniela Delneri and Raymond T. O’Keefe1 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, U.K. Abstract New sequencing technologies and high-resolution microarray analysis have revealed genome-wide pervasive transcription in many eukaryotes, generating a large number of RNAs with no coding capacity. The focus of current debate is whether many of these ncRNAs (non-coding RNAs) are functional, and if so, what their function is. In this review, we describe recent discoveries in the field of ncRNAs in the yeast Saccharomyces cerevisiae. Newly identified ncRNAs in this budding yeast, their functions in gene regulation and possible mechanisms of action are discussed. The development of new technologies, including high- resolution tiling arrays and second-generation sequencing (e.g. RNA-Seq), has revealed that a large fraction of the eukaryotic genome is transcribed, even in areas previously thought to be transcriptionally inactive, such as non-coding regions [1,2]. While providing us with novel information about genome-wide occupancy of the transcriptional ma- chinery, these observations also raise a major question as to whether the RNAs transcribed from these regions have any biological functions or are they just transcriptional noise that is ultimately degraded by the RNA surveillance pathways. Recent studies from a variety of eukaryotic organisms from yeast to humans have demonstrated that at least transcription of a subset of ncRNAs (non-coding RNAs), especially those nearby protein-coding genes, play a role in transcription regulation [3–5]. The aim of this review is to provide a recent snapshot in the field of ncRNAs in the first sequenced eukaryote Saccharomyces cerevisiae. We will focus here on newly identified ncRNAs in this budding yeast, their functions in gene regulation and possible mechanisms of action. ncRNAs identif

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Non-coding RNAs in Saccharomyces cerevisiae: what...

on Biochemical Society Transactio... August 2012

[In vivo functions of long non-coding RNAs].

on Yi chuan = Hereditas / Zhonggu... March 2014

Life without RNAi: noncoding RNAs and their functi...

on Biochemistry and Cell Biology October 2009
More articles like this..