Abstract The chromatographic behavior of neutral lipids on chromarods, used for the Iatroscan TH-10 analyzer, was evaluated and compared to that observed in adsorption thin-layer chromatography on silica gel. Various proportions of hexane, diethyl ether and formic acid were used in the developing solvent to determine changes in R F values of the neutral lipid classes. In addition, acetic and propionic acids were investigated as substitutes for formic acid in the developing solvent. A knowledge of the chromatographic behavior of the neutral lipid classes, with systematic changes in the concentration of individual solvents in the developing solvent mixture, allows maximum resolution to be obtained. The R F values of some lipid components change with extensive use of the chromarods (25 to 30 uses); however, the life of the chromarods can be extended by changing the proportion of the solvent components in accordance with the chromatographic characteristics determined in this study.