Affordable Access

Publisher Website

Effect of solvents on the resolution of neutral lipids on chromarods

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
198
Issue
3
Identifiers
DOI: 10.1016/s0021-9673(00)84767-6

Abstract

Abstract The chromatographic behavior of neutral lipids on chromarods, used for the Iatroscan TH-10 analyzer, was evaluated and compared to that observed in adsorption thin-layer chromatography on silica gel. Various proportions of hexane, diethyl ether and formic acid were used in the developing solvent to determine changes in R F values of the neutral lipid classes. In addition, acetic and propionic acids were investigated as substitutes for formic acid in the developing solvent. A knowledge of the chromatographic behavior of the neutral lipid classes, with systematic changes in the concentration of individual solvents in the developing solvent mixture, allows maximum resolution to be obtained. The R F values of some lipid components change with extensive use of the chromarods (25 to 30 uses); however, the life of the chromarods can be extended by changing the proportion of the solvent components in accordance with the chromatographic characteristics determined in this study.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Quantitative determination of neutral lipids on ch...

on Journal of Chromatography A Jan 01, 1982

[Effect of flavonoids of different structure on pe...

on Prikladnaia biokhimiia i mikro... 2005

Neutral lipids of barley grain.

on Journal of Agricultural and Fo... 1980
More articles like this..