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Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

Authors
Journal
Genome Biology
1465-6906
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
5
Issue
10
Identifiers
DOI: 10.1186/gb-2004-5-10-r82
Keywords
  • Method

Abstract

Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.

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