Affordable Access

Collection and characterisation of bacterial membrane proteins

Authors
Publisher
Elsevier
Publication Date
Keywords
  • Q Science (General)
  • Qc Physics
  • Qd Chemistry
Disciplines
  • Biology

Abstract

A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative alpha-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Bacterial membrane proteins.

on Microbiological sciences April 1987

Assembly of bacterial inner membrane proteins.

on Annual Review of Biochemistry 2011
More articles like this..