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Novel method for determination of myofibril fragmentation post-mortem

Authors
Journal
Meat Science
0309-1740
Publisher
Elsevier
Publication Date
Volume
75
Issue
4
Identifiers
DOI: 10.1016/j.meatsci.2006.10.002
Keywords
  • Myofibril Fragmentation
  • Mfi
  • Post-Mortem Changes
  • Myofibril Particle Distribution
  • Warner–Bratzler Shear Force
  • Pork

Abstract

Abstract Multi angle light scattering was used to determine the myofibril fragmentation of pig longissimus dorsi muscle which was then compared with results from the common turbidity method. The method is based on measurement of the myofibril particle size distribution with the use of a special optical unit containing several individual detectors. The method was able to determine post-mortem changes in a pig muscle homogenate without purification of the myofibrils and is therefore simpler and much faster than the traditional turbidity method. There was a significant correlation ( p < 0.01) between Warner–Bratzler shear force (WBSF) and particle size distribution. The root mean square error of prediction was found to be 6.1 N (10–15% of the measured WBSF) when multivariate data analysis was used to make a prediction model for WBSF. Multi angle light scattering is very useful for estimation of myofibril fragmentation since the method is fast and the sample preparation is simple.

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