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Succinyl-CoA synthetase in greening maize leaves

Authors
Journal
Phytochemistry
0031-9422
Publisher
Elsevier
Publication Date
Volume
14
Issue
10
Identifiers
DOI: 10.1016/s0031-9422(00)91090-2
Keywords
  • Zea Mays
  • Gramineae
  • Maize
  • Succinyl-Coa Synthetase
  • Greening.
Disciplines
  • Biology

Abstract

Abstract In extracts of greening maize leaves succinyl-CoA synthetase was present in both a particulate and a soluble fraction. Aqueous and non-aqueous fractionation together with determination of chlorophyll content and cytochrome oxidase activity indicated that the enzyme was neither located, nor originated in plastids. Pre-illumination of leaves caused only small increases in the activity of either the particulate or the soluble enzyme. The soluble enzyme was ATP specific and had a low affinity for succinate ( Km = 63 mM).

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