Affordable Access

Candida albicans- adriamycin interactions: ultrastructural and spectrofluorometric study of whole yeasts and spheroplasts

Authors
Journal
Biology of the Cell
0248-4900
Publisher
Wiley Blackwell (Blackwell Publishing)
Publication Date
Keywords
  • Candida
  • Adriamycin
  • Nucleus
  • Nucleolus
Disciplines
  • Medicine

Abstract

Summary The occurrence of candidiasis in cancer patients who undergo chemotherapy requires the interrelation of Candida albicans and the antimitotic drug Adriamycin (ADM) which is well known as an intercalating agent. The whole yeasts were not affected by 2 h of contact with the drug at 10 -4 M neither for their growth curve nor for their ultrastructure, despite the presence of free ADM on their surface. Spheroplasts displayed a delay in their growth and exhibited altered nucleoli with segregation of their granular and fibrillar components. The modified emission spectrum of ADM, determined by spectrofluorometry, corresponded neither to the free ADM nor to the DNA-bound drug, but it could be related to a metabolite of the drug. The cell wall appeared to be one of the main sites for ADM resistance of Candida albicans in vitro.

There are no comments yet on this publication. Be the first to share your thoughts.