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Multiplex NMR: A novel methodology for high -throughput NMR analysis

Authors
Publisher
Purdue University
Publication Date
Keywords
  • Chemistry
  • Analytical|Engineering
  • Biomedical
Disciplines
  • Chemistry

Abstract

In this thesis, multiplex NMR methodology is described, which has the potential to significantly increase the throughput of NMR measurements. This novel methodology has possible applications in areas where large numbers of samples need to be analyzed. Multiple-coil NMR probes have been constructed to analyze multiple samples simultaneously using parallel detecting microcoils that were incorporated into a single RF circuit. Linear field gradients generated either by offsetting Z1 shim from its optimal value or by pulsed field gradient coils were used to differentiate signals from different coils. Both one-dimensional and two-dimensional NMR experiments have been carried out. Spectral analysis strategy, rapid selective excitation and chemical shift imaging methods have been developed to obtain well-resolved sub-spectra of samples in different NMR coils. Issues of quantitation, resolution, limit of detection and solvent suppression capabilities of the multiplex NMR probes were also investigated. In order to further improve the throughput of NMR analysis, the possibility of realizing automated multiple-sample NMR detection using the multiplex NMR probe is also studied. Direct sample injection, flow injection analysis controlled sample injection, and a Gilson-215 automatic liquid handler have been incorporated with the multiplex NMR probe to realize the controlled, high speed flow introduction of multiple samples into the NMR probe, and rapid selective excitation method has been utilized to analyze the multiple samples. As a result, the time needed to analyze each sample is greatly reduced. ^

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