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Rapid structural characterization of triterpenoid saponins in crude extract from Symplocos chinensisusing liquid chromatography combined with electrospray ionization tandem mass spectrometry

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1101
Identifiers
DOI: 10.1016/j.chroma.2005.09.058
Keywords
  • Symplocos Chinensis
  • Triterpenoid Saponins
  • Analytical Method
  • Positive And Negative Ion Esi–Msn
  • Lc–Msn

Abstract

Abstract Triterpenoid saponins in bioactive crude extract from S ymplocos chinensis were rapidly identified using electrospray ionization multi-stage tandem mass spectrometry (ESI–MS n ) and liquid chromatography coupled with sequential mass spectrometry (LC–MS n ). According to the characteristic fragmentation behavior of known glucuronide-type triterpenoid saponins isolated from this plant, a total of fourteen constituents in the crude extract were structurally characterized on the basis of their retention time and tandem mass spectrometric analysis, including five pairs of naturally occurring isomers. Except one known saponin formerly obtained, the other constituents were new compounds. The analytical method of LC–MS n combined with ESI–MS n in positive and negative ion modes has been developed for the direct structural elucidation of triterpenoid saponins of this kind in plant extracts.

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