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Enzymatic determination of nicotinamide adenine dinucleotide phosphate with a silver sulfide membrane electrode

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
151
Identifiers
DOI: 10.1016/s0003-2670(00)80110-5

Abstract

Abstract A simple, sensitive and selective method is proposed for the determination of nicotinamide adenine dinucleotide phosphate (NADPH) in the concentration range 10 −4–10 −7 mol l −1 (90 μg—90 ng ml −1). The method is based on monitoring with a silver sulfide membrane electrode the concentration of the reduced glutathione released at pH 8 by reaction of oxidized glutathione with NADPH in the presence of glutathione reductase. Calibration data for 10 −4–10 −7 mol l −1 range of NADPH yielded a least-squares equation of (Δ E, mV) = (36.65 ± 0.15) log C + 264.93 ± 0.1 with a standard error of 0.4 mV. Other common nucleotides including NADP + do not interfere.

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