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Phytochrome models:V. Chromatographic detection of solvent adducts of bilinoid pigments

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
205
Issue
1
Identifiers
DOI: 10.1016/s0021-9673(00)81816-6

Abstract

Abstract The dimethyl esters of biliverdin, its XIIIα isomer (mesobiliverdin) and bilirubin and the 7,13-dimethyl-2,3,8,12,17,18-hexaethyl- and 2,3-dihydrooctaethylbilitrienes form strong adducts with solvents such as tetrahydrofuran, acetonitrile and ethyl lactate. These are differently adsorbed on silica gel. They give rise to two peaks in high-performance liquid chromatography and are separated into two zones on preparative low-pressure column chromatography. As chromatographic methods are normally used to assess the purity of bilinoid pigments, the results represent a warning regarding the solvent systems chosen in high-performance liquid and thin-layer chromatographic analyses.

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