Affordable Access

Protocol for Rapid Fluorescence In Situ Hybridization of Bacteria in Cryosections of Microarthropods

Authors
Publisher
American Society for Microbiology
Publication Date
Source
PMC
Keywords
  • Methods

Abstract

A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and reduces the number of manipulations which might damage the microscopic material. The method allowed the study of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria in both the gut and tissue.

There are no comments yet on this publication. Be the first to share your thoughts.