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Serum induces the immediate opening of Ca2+-activated channels in quiescent human fibroblasts

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
241
Identifiers
DOI: 10.1016/0014-5793(88)81052-4
Keywords
  • Serum
  • Cell Activation
  • Calcium Mobilization
  • Membrane Current
  • (Fibroblast)

Abstract

Abstract Application of fetal calf serum to quiescent human fibroblasts produces an immediate (3 – 20 s delay) increase in membrane conductance which lasts about 20 – 30 s. This conductance is strongly outwardly-rectifying and has a reversal potential between −45 and −10 mV. The conductance increase may also be induced by application of the Ca 2+ ionophore A23187 while it does not occur when intracellular K + is replaced by Cs +. It is concluded that this early effect of serum is due to the opening of Ca 2+-activated channels. This permeability change will alter the membrane potential and thus possibly interact with other voltage-sensitive processes induced by serum growth factors.

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