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Impact of urea on water structure: a clue to its properties as a denaturant?

Authors
Journal
Biophysical Chemistry
0301-4622
Publisher
Elsevier
Publication Date
Volume
105
Identifiers
DOI: 10.1016/s0301-4622(03)00095-4
Keywords
  • Neutron Diffraction
  • Isotope Substitution
  • Protein Denaturation
  • Hydration
Disciplines
  • Biology

Abstract

Abstract A new investigation of the structure of urea–water solutions at a mole ratio of 1 urea to 4 water molecules is described. Neutron diffraction is used in conjunction with isotope labelling on the water and urea hydrogen atoms and on the nitrogen atom of urea. The diffraction data are analysed using the empirical potential structure refinement procedure to yield a set of site–site radial distribution functions and spatial density functions that are consistent with the diffraction data. The results are discussed in relation to recent and past X-ray and neutron diffraction experiments and theoretical studies of this system. It is found that urea incorporates readily into water, forming pronounced hydrogen bonds with water at both the amine and carbonyl headgroups. In addition the urea also hydrogen bonds to itself, forming chains or clusters consisting of up to approximately 60 urea molecules in a cluster. There, is however, little or no evidence of urea segregating itself from water, in marked contrast to a recent study of the methanol–water system. This behaviour is discussed in the context of the great propensity of urea to effect protein denaturation.

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