Affordable Access

Publisher Website

On the application of Job's method of continuous variation to the stoichiometry of protein-ligand complexes

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
68
Issue
2
Identifiers
DOI: 10.1016/0003-2697(75)90666-1
Disciplines
  • Biology

Abstract

Abstract The application of the method of continuous variation to protein-ligand complexes is critically examined for the case of a nonassociating protein which has n equivalent and independent ligand-binding sites. It is shown that in order to obtain a reliable estimate of the stoichiometry, the sum of protein and ligand concentrations must be kept large relative to the dissociation constant.

There are no comments yet on this publication. Be the first to share your thoughts.