Affordable Access

The cellular biology of flexor tendon adhesion formation: an old problem in a new paradigm.

Authors
Journal
American Journal Of Pathology
0002-9440
Publisher
Elsevier
Publication Date
Disciplines
  • Biology
  • Chemistry

Abstract

Intrasynovial flexor tendon injuries of the hand can frequently be complicated by tendon adhesions to the surrounding sheath, limiting finger function. We have developed a new tendon injury model in the mouse to investigate the three-dimensional cellular biology of intrasynovial flexor tendon healing and adhesion formation. We investigated the cell biology using markers for inflammation, proliferation, collagen synthesis, apoptosis, and vascularization/myofibroblasts. Quantitative immunohistochemical image analysis and three-dimensional reconstruction with cell mapping was performed on labeled serial sections. Flexor tendon adhesions were also assessed 21 days after wounding using transmission electron microscopy to examine the cell phenotypes in the wound. When the tendon has been immobilized, the mouse can form tendon adhesions in the flexor tendon sheath. The cell biology of tendon healing follows the classic wound healing response of inflammation, proliferation, synthesis, and apoptosis, but the greater activity occurs in the surrounding tissue. Cells that have multiple "fibripositors" and cells with cytoplasmic protrusions that contain multiple large and small diameter fibrils can be found in the wound during collagen synthesis. In conclusion, adhesion formation occurs due to scarring between two damaged surfaces. The mouse model for flexor tendon injury represents a new platform to study adhesion formation that is genetically tractable.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

The cellular biology of flexor tendon adhesion for...

on American Journal Of Pathology Nov 01, 2009

The Cellular Biology of Flexor Tendon Adhesion For...

on American Journal Of Pathology Jan 01, 2009

The cellular biology of flexor tendon adhesion for...

on American Journal Of Pathology Nov 01, 2009

Advances in the biology of zone II flexor tendon h...

on Annals of plastic surgery July 2000
More articles like this..