Affordable Access

Bioactivity of nitrolinoleate: effects on adhesion molecules and CD40-CD40L system

Authors
Journal
The Journal of Nutritional Biochemistry
0955-2863
Publisher
Elsevier
Publication Date
Keywords
  • Intravital Microscopy
  • Adhesion Molecules
  • Cd40-Cd40L System
  • Nitrated Lipids
  • Linoleic Acid
Disciplines
  • Biology

Abstract

The vascular effects of nitrolinoleate (LNO(2)), an endogenous product of linoleic acid (LA) nitration by nitric oxide-derived species and a potential nitrosating agent, were investigated on rat endothelial-leukocyte interactions. Confocal microscopy analysis demonstrated that LNO(2) was capable to deliver free radical nitric oxide ((center dot)NO) into cells, 5 min after its administration to cultured cells, with a peak of liberation at 30 min. THP-1 monocytes incubated with LNO(2) for 5 min presented nitrosation of CD40, leading to its inactivation. Other anti-inflammatory actions of LNO(2) were observed in vivo by intravital microscopy assays. LNO(2) decreased the number of adhered leukocytes in postcapillary venules of the mesentery network. In addition to this, LNO(2) reduced mRNA and protein expression of 2-integrin in circulating leukocytes, as well as VCAM-1 in endothelial cells isolated from postcapillary venules, confirming its antiadhesive effects on both cell types. Moreover, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, a nitric oxide scavenger, partially abolished the inhibitory action of LNO(2) on leukocyte-endothelium interaction, suggesting that the antiadhesion effects of LNO(2) involve a dual role in leukocyte adhesion, acting as a nitric oxide donor as well as through nitric oxide-independent mechanisms. In conclusion, LNO(2) inhibited adhesion molecules expression and promoted (center dot)NO inactivation of the CD40-CD40L system, both important processes of the inflammatory response. (C) 2010 Elsevier Inc. All rights reserved.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Bioactivity of nitrolinoleate: effects on adhesion...

on The Journal of Nutritional Bio... Jan 01, 2010

CD40/CD40L system and vascular disease.

on Internal and emergency medicin... December 2007

Pairs of surface molecules involved in human IgE r...

on The European respiratory journ... August 1996
More articles like this..