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Flow-injection determination of trace amounts of dopamine by chemiluminescence detection

Authors
Journal
Talanta
0039-9140
Publisher
Elsevier
Publication Date
Volume
50
Issue
3
Identifiers
DOI: 10.1016/s0039-9140(99)00164-2
Keywords
  • Chemiluminescence
  • Dopamine
  • Flow-Injection
  • Lucigenin

Abstract

Abstract A flow-injection analysis (FIA) for the determination of dopamine has been developed. The method is based on the inhibition effect of dopamine on the iron(II)-induced chemiluminescence (CL) of 10,10′-dimethyl-9,9′-biacridinium dinitrate (lucigenin). The presence of a non-ionic surfactant, polyoxyethylene (23) lauryl ether (Brij 35), caused an increase in the inhibition effect. The present method allows the determination of dopamine over the range 1×10 −8–2×10 −7 mol dm −3. The relative standard deviation was 0.7% for eight determinations of 6×10 −8 mol dm −3 dopamine. The detection limit ( S/ N=3) was 2×10 −9 mol dm −3 with the sampling rate of 40 samples h −1. The effect of other catecholamines and compounds of similar structure on the lucigenin CL reaction was studied: quinone, hydroquinone, norepinephrine, pyrocatechol and l-dopa suppressed the CL intensity.

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