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Rapid and accurate pyrosequencing of angiosperm plastid genomes

BioMed Central
  • Research Article

Abstract ral ss BioMed CentBMC Plant Biology Open AcceResearch article Rapid and accurate pyrosequencing of angiosperm plastid genomes Michael J Moore*1,2, Amit Dhingra3, Pamela S Soltis2, Regina Shaw4, William G Farmerie4, Kevin M Folta3 and Douglas E Soltis1 Address: 1Department of Botany, University of Florida, P.O. Box 118526, Gainesville, FL, 32611, USA, 2Florida Museum of Natural History, University of Florida, P.O. Box 117800, Gainesville, FL, 32611, USA, 3Horticultural Sciences Department, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA and 4ICBR Genome Sequencing Service Laboratory, University of Florida, P.O. Box 100156, Gainesville, FL, 32610, USA Email: Michael J Moore* - [email protected]; Amit Dhingra - [email protected]; Pamela S Soltis - [email protected]; Regina Shaw - [email protected]; William G Farmerie - [email protected]; Kevin M Folta - [email protected]; Douglas E Soltis - [email protected] * Corresponding author Abstract Background: Plastid genome sequence information is vital to several disciplines in plant biology, including phylogenetics and molecular biology. The past five years have witnessed a dramatic increase in the number of completely sequenced plastid genomes, fuelled largely by advances in conventional Sanger sequencing technology. Here we report a further significant reduction in time and cost for plastid genome sequencing through the successful use of a newly available pyrosequencing platform, the Genome Sequencer 20 (GS 20) System (454 Life Sciences Corporation), to rapidly and accurately sequence the whole plastid genomes of the basal eudicot angiosperms Nandina domestica (Berberidaceae) and Platanus occidentalis (Platanaceae). Results: More than 99.75% of each plastid genome was simultaneously obtained during two GS 20 sequence runs, to an average depth of coverage of 24.6× in Nandina and 17.3× in Platanus. The Nandina and Platanus plastid genomes shared essentially identical gene

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