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Cloning of a G protein-activated inwardly rectifying potassium channel from human cerebellum

Authors
Journal
Molecular Brain Research
0169-328X
Publisher
Elsevier
Publication Date
Volume
39
Identifiers
DOI: 10.1016/0169-328x(95)00349-w
Keywords
  • Inward Rectifier
  • Potassium Channel
  • G Protein
  • Cdna Cloning
  • Human Cerebellum
  • Restriction Fragment Length Polymorphism
  • Serotonin Receptor
Disciplines
  • Biology

Abstract

Abstract Based on sequence homology with the rat atrial G protein-coupled muscarinic potassium channel (GIRK1 or KGA1/KGB1), a human cDNA encoding a G protein-activated inwardly rectifying K + channel (HGIRK1) was isolated. The cDNA encodes a protein of 501 amino acids and shares 99% identity to rat GIRK1 in its total amino acid sequence. Southern blot analysis of genomic DNA indicates a high degree of conservation among various species. In the human population of useful NlaIII restriction fragment length polymorphism was found in the coding sequence of HGIRK1. Co-expression of HGIRK1 and the 5-HT1A receptor in Xenopus oocytes resulted in opening of the channel upon treatment with serotonin. HGIRK1 currents showed strong inward rectification and could be blocked by extracellular Ba 2+. Northern blot analysis shows that HGIRK1 expression in human is most abundant in the brain, while lower levels are found in kidney and heart.

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