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The chitinase encoding Tn7-basedchiAgene endowsPseudomonas fluorescenswith the capacity to control plant pathogens in soil

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
147
Issue
1
Identifiers
DOI: 10.1016/0378-1119(94)90042-6
Keywords
  • Transposon
  • Recombinant Dna
  • Serratia
  • Rhizoctonia
  • Ptacpromoter
Disciplines
  • Biology

Abstract

Abstract A transposon system based on Tn7 carrying the chiA gene (encoding chitinase) of Serratia marcescens was established. The transposon was introduced by conjugation into Pseudomonas fluorescens as a stable integrate expressing and secreting the active chitinase enzyme. The resulting strain was shown to control the plant pathogen Rhizoctonia solani in soil.

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